Advantages of RPPX Columns
- Designed for LC/MS mobile phase (Acetonitrile: Water: Acetic Acid). Unlike Sodium Hydroxide base mobile phases which can contaminate your LC/MS, this mobile phase system will ensure that your LC/MS system is in good working conditions.
- Designed for high organic solvent elution to maximize the ionization in LC/MS source. This property will ensure great ionization of ClO4 in LC/MS electro spray source. In general for LC/MS mobile phase, the more organic solvent content the better ionization you will get because the LC/MS source can evaporate away the mobile phase faster.
- Designed for high organic solvent elution to minimize column contaminations. Because many real world samples will contain some types of organic compounds, RPPX series columns are designed to be run at the working condition of about 50% Acetonitrile: 50% dH2O. At this level of organic solvent, the column packing does not have abilities to retain organic molecules. Thus, organic molecules from very complex samples like waste water, soil extract, biological liquid (milk), and plant extract will just elute very close to the void volume of the column. Hence, the optima operating mobile phase of this column is the same as the cleaning conditions for organic contaminants.
- Designed selectively to retain Perchlorate and not other ions. Unlike Ion Exchange base columns, RPPX series columns are designed without Ion Exchange site to retain ions and therefore will not be subjected to any ions contaminants and co-elution like Sulfate. Taking advantage of the hydrophobic property of Perchlorate, RPPX columns have good selectivity for Perchlorate and other ions alike like Chlorate (ClO3). See test chromatogram.
- Retention time for Perchlorate is depending on the composition content of Acetonitrile in mobile phase. The more Acetonitrile concentration in mobile phase the longer the retention time. This property is exceptionally good for LC/MS applications since more organic solvent is better of LC/MS ionization.
Cleaning and Storage:
- 80% ACN: 20% H2O solution is recommended for both cleaning and storage.
- For long term storage the column should be flushed with the storage solution every 3 months to make sure that the column packing material will not dry out.
Depending on the flow requirement of your LC/MS or MS/MS system, RPPX columns are available in 3 different options:
- 4.6 x 250 mm 5.0 um particles – recommended flow rate 0.40 - 0.50 ml/min
- 3.0 x 150 mm 3.5 um particles – recommended flow rate 0.25 – 0.30 ml/min
- 2.1 x 150 mm 5.0 um particles – recommended flow rate 0.15 – 0.20 ml/min